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This five day Skills course is suitable for MSD postgraduate research students and early career researchers wanting to learn about NMR via lectures and practical sessions.

Please note this course takes place over five days and participants will be expected to attend all sessions:

The NMR course consists of lectures and practical sessions. No prior knowledge of NMR is assumed. For further information contact (christina.redfield@bioch.ox.ac.uk)

  

Monday February 10th 9:30am - 5pm

Introduction to Biomolecular NMR and Assignment of Protein NMR Spectra

  

Tuesday February 11th 9:30am - 5pm

Assignment of Protein NMR Spectra

Protein Structure Determination by NMR

  

Wednesday February 12th 9:30am - 5pm

NMR of Intrinsically Disordered Proteins 

NMR of Nucleic Acids

Protein Dynamics

  

Thursday February 13th 9:30am - 5pm

NMR of Oligosaccharides

Biological Solid-state NMR

Protein-ligand Interactions by NMR

  

Friday February 14th 9:30am - 1pm

Solid-state NMR for Protein Structure Determination

Solution NMR of Membrane Proteins

 

COURSE AIMS

The course will focus on the application of solution-state NMR to the study of different types of biomolecules. The emphasis is on solution-state NMR studies of proteins but applications to nucleic acids and oligosaccharides will also be presented. In addition, more advanced topics including residual dipolar couplings, dynamics, solid-state biomolecular NMR and protein-ligand interations will be introduced.

  

COURSE FORMAT

Topics to be covered include:

 

1) Introduction to Biomolecular NMR

2) Assignment of Protein NMR Spectra

3) Assignment using 15N and 13C Labeling

4) Data Harvesting and Structure Determination I

5) Residual Dipolar Couplings

6) Data Harvesting and Structure Determination II

7) Protein Dynamics

8) Using NMR to study Intrinsically Disordered Proteins

9) Using NMR Spectroscopy to Study Protein Folding and Partially Folded Proteins

10) Protein-ligand interactions by NMR

11) Solution NMR Studies of Membrane Proteins

12) Principles and applications of biological solid state NMR

13) Solid-state NMR for protein structure determination

14) NMR of Nucleic Acids

15) Use of NMR for identification and conformational determination of oligosaccharides and related alkaloids

  

COURSE LENGTH 

The course will run for 5 days and include lectures and practical sessions.

  

PARTICIPANT NUMBERS 

Maximum 25