This course is scheduled once a year in Hilary term.
Please note this course takes place over five days and participants will be expected to attend all sessions:
The NMR course consists of lectures and practical sessions. No prior knowledge of NMR is assumed. For further information contact (christina.redfield@bioch.ox.ac.uk)
Monday February 4th 9:30am - 5pm
Introduction to Biomolecular NMR and Assignment of Protein NMR Spectra
Tuesday February 5th 9:30am - 5pm
Assignment of Protein NMR Spectra
Protein Structure Determination by NMR
Wednesday February 6th 9:30am - 5pm
NMR of Intrinsically Disordered Proteins
NMR of Nucleic Acids
Protein Dynamics
Thursday February 7th 9:30am - 5pm
NMR of Oligosaccharides
Biological Solid-state NMR
Protein-ligand Interactions by NMR
Friday February 8th 9:30am - 1pm
Solid-state NMR for Protein Structure Determination
Solution NMR of Membrane Proteins
COURSE AIMS
The course will focus on the application of solution-state NMR to the study of different types of biomolecules. The emphasis is on solution-state NMR studies of proteins but applications to nucleic acids and oligosaccharides will also be presented. In addition, more advanced topics including residual dipolar couplings, dynamics, solid-state biomolecular NMR and protein-ligand interations will be introduced.
COURSE FORMAT
Topics to be covered include:
1) Introduction to Biomolecular NMR
2) Assignment of Protein NMR Spectra
3) Assignment using 15N and 13C Labeling
4) Data Harvesting and Structure Determination I
5) Residual Dipolar Couplings
6) Data Harvesting and Structure Determination II
7) Protein Dynamics
8) Using NMR to study Intrinsically Disordered Proteins
9) Using NMR Spectroscopy to Study Protein Folding and Partially Folded Proteins
10) Protein-ligand interactions by NMR
11) Solution NMR Studies of Membrane Proteins
12) Principles and applications of biological solid state NMR
13) Solid-state NMR for protein structure determination
14) NMR of Nucleic Acids
15) Use of NMR for identification and conformational determination of oligosaccharides and related alkaloids
COURSE LENGTH
The course will run for 5 days and include lectures and practical sessions.
PARTICIPANT NUMBERS
Maximum 25