NMR - ONLINE

You are expected to attend four sessions:

8 February 2021 @ 9:30 - 17:00

9 February 2021 @ 9:30 - 17:00

10 February 2021 @ 9:30 - 17:00

11 February 2021 @ 9:30 - 17:00

COURSE AIM

The course will focus on the application of solution-state NMR to the study of different types of biomolecules. The emphasis is on solution-state NMR studies of proteins but applications to nucleic acids and oligosaccharides will also be presented. In addition, more advanced topics including residual dipolar couplings, dynamics, solid-state biomolecular NMR and protein-ligand interations will be introduced.

 COURSE OBECTIVES

1) To gain an understanding of the types of useful information that can be obtained from nuclear magnetic resonance (NMR) spectroscopy data of biomolecules including proteins and nucleic acids.

2) To gain a basic understanding of the principles of 1D and multidimensional NMR spectroscopy.

3) To gain an understanding of how NMR data are collected and how NMR spectra are analysed in order to extract information about structure, dynamics and interactions of biomolecules.

3) To gain an understanding of the limitations of NMR spectroscopy and the types of systems that are likely to be suitable for NMR.

COURSE FORMAT

Topics to be covered include:

1) Introduction to Biomolecular NMR
2) Assignment of Protein NMR Spectra
3) Assignment using 15N and 13C Labeling
4) Data Harvesting and Structure Determination I
5) Residual Dipolar Couplings
6) Data Harvesting and Structure Determination II
7) Protein Dynamics
8) Using NMR to study Intrinsically Disordered Proteins
9) Using NMR Spectroscopy to Study Protein Folding and Partially Folded Proteins
10) Protein-ligand interactions by NMR
11) Solution NMR Studies of Membrane Proteins
12) Principles and applications of biological solid state NMR
13) Solid-state NMR for protein structure determination
14) NMR of Nucleic Acids
15) Use of NMR for identification and conformational determination of oligosaccharides and related alkaloids

PARTICIPANT NUMBERS

25