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This interactive online course for MSD postgraduate research staff and students consists of lectures and practical sessions. No prior knowledge of NMR is assumed.

You are expected to attend four sessions:

8 February 2021 @ 9:30 - 17:00

9 February 2021 @ 9:30 - 17:00

10 February 2021 @ 9:30 - 17:00

11 February 2021 @ 9:30 - 17:00

COURSE AIM

The course will focus on the application of solution-state NMR to the study of different types of biomolecules. The emphasis is on solution-state NMR studies of proteins but applications to nucleic acids and oligosaccharides will also be presented. In addition, more advanced topics including residual dipolar couplings, dynamics, solid-state biomolecular NMR and protein-ligand interations will be introduced.

 COURSE OBECTIVES

1) To gain an understanding of the types of useful information that can be obtained from nuclear magnetic resonance (NMR) spectroscopy data of biomolecules including proteins and nucleic acids.

2) To gain a basic understanding of the principles of 1D and multidimensional NMR spectroscopy.

3) To gain an understanding of how NMR data are collected and how NMR spectra are analysed in order to extract information about structure, dynamics and interactions of biomolecules.

3) To gain an understanding of the limitations of NMR spectroscopy and the types of systems that are likely to be suitable for NMR.

COURSE FORMAT

Topics to be covered include:

1) Introduction to Biomolecular NMR
2) Assignment of Protein NMR Spectra
3) Assignment using 15N and 13C Labeling
4) Data Harvesting and Structure Determination I
5) Residual Dipolar Couplings
6) Data Harvesting and Structure Determination II
7) Protein Dynamics
8) Using NMR to study Intrinsically Disordered Proteins
9) Using NMR Spectroscopy to Study Protein Folding and Partially Folded Proteins
10) Protein-ligand interactions by NMR
11) Solution NMR Studies of Membrane Proteins
12) Principles and applications of biological solid state NMR
13) Solid-state NMR for protein structure determination
14) NMR of Nucleic Acids
15) Use of NMR for identification and conformational determination of oligosaccharides and related alkaloids

PARTICIPANT NUMBERS

25

HOW IT WILL WORK

More information will be provided closer to the time to registered participants. Prof. Christina Redfield will give a live talk "An Introduction to Biomolecular NMR" talk on 8 February at 9:30-10:30 explaining the course format. For further information contact christina.redfield@bioch.ox.ac.uk.

WHAT YOU WILL NEED

A good internet connection, uninterrupted time, camera and microphone enabled on your PC.

ATTENDANCE CERTIFICATE ON SURVEY COMPLETION

It is now a requirement that you complete the three short questions in the survey you receive after attending the course. Once you have submitted the survey, you will be sent an email with a link to your attendance certificate. This is to ensure we receive the feedback we need to evaluate and improve our courses. Survey results are downloaded and stored anonymously.

PLEASE NOTE

Where there is no cost in the shopping trolley, no deposit is required. However, two or more non-attendances or late cancellations without good reason will be logged and may mean you cannot attend any further MSD training that term. Please refer to our Terms and Conditions for further information.