Electron cryomicroscopy (cryo-EM) and tomography of bacterial biofilms
Our laboratory uses electron cryomicroscopy (cryo-EM), tomography (cryo-ET) along with associated image processing techniques to study biofilm formation of pathogenic bacteria like Pseudomonas aeruginosa and Escherichia coli. Correlated light and electron microscopy (CLEM) coupled with mass spectrometry (MS) are used to support our investigations. We combine in vitro reconstitution of key molecules with in vivo imaging to understand how bacteria form biofilms. Please contact Tanmay Bharat if you are interested in our work or visit our page on the Dunn School website for more details.
Bharat, T.A.M., Ciziene, D.K., Hardy, G.G., Yu, E.W., Devant, J.M., Hagen, W.J., Brun, Y.V., Briggs, J.A., and Löwe, J (2017) Structure of the hexagonal surface layer on Caulobacter crescentus cells. Nature Microbiology, 2 17059
Bharat, T. A., Murshudov, G. N., Sachse, C., and Löwe, J. (2015) Structures of actin-like ParM filaments show architecture of plasmid-segregating spindles. Nature 523, 106-110
Bharat, T. A., Russo, C. J., Löwe, J., Passmore, L. A., and Scheres, S. H. (2015) Advances in Single-Particle Electron Cryomicroscopy Structure Determination applied to Sub-tomogram Averaging. Structure 23, 1743-1753
Bharat, T. A., Castillo Menendez, L. R., Hagen, W. J., Lux, V., Igonet, S., Schorb, M., Schur, F. K., Kräusslich, H. G., and Briggs, J. A. (2014) Cryo-electron microscopy of tubular arrays of HIV-1 Gag resolves structures essential for immature virus assembly. Proc Natl Acad Sci U S A 111, 8233-8238
Bharat, T. A., Davey, N. E., Ulbrich, P., Riches, J. D., de Marco, A., Rumlova, M., Sachse, C., Ruml, T., and Briggs, J. A. (2012) Structure of the immature retroviral capsid at 8 Å resolution by cryo-electron microscopy. Nature 487, 385-389