Associate Professor of Cell Biology
DNA replication and genome stability
A primary aim of our group is to understand how eukaryotic genomes are completely and accurately replicated. Errors during DNA replication give rise to mutations that cause genetic disease; failures during genome replication directly underlie several human disorders; and DNA replication is the direct target of many chemotherapeutic agents.
Eukaryotic genomes are replicated from multiple discrete initiation sites, called replication origins. Replication is controlled primarily by the regulated activation of origins. Failure to activate sufficient or appropriately distributed origins can result in unreplicated regions of the genome, which cannot be properly segregated during cell division. Furthermore, origins activate at characteristic times during S phase, resulting in genomes replicating in a highly conserved, characteristic temporal order. This is of critical importance, as illustrated by disruption of replication timing in cancer cells, which contributes to genome instability by leading to chromosome breaks, translocations and aneuploidy.
Further details can be found in our recent published work. Please get in touch if you would like to discuss or develop a project opportunity within the Nieduszynski group.