NMR

Please note this course takes place over five days and participants will be expected to attend all sessions:

The NMR course consists of lectures and practical sessions. No prior knowledge of NMR is assumed. For further information contact (christina.redfield@bioch.ox.ac.uk)

Monday February 4th 9:30am - 5pm

Introduction to Biomolecular NMR and Assignment of Protein NMR Spectra

Tuesday February 5th 9:30am - 5pm

Assignment of Protein NMR Spectra

Protein Structure Determination by NMR

Wednesday February 6th 9:30am - 5pm

NMR of Intrinsically Disordered Proteins 

NMR of Nucleic Acids

Protein Dynamics

Thursday February 7th 9:30am - 5pm

NMR of Oligosaccharides

Biological Solid-state NMR

Protein-ligand Interactions by NMR

Friday February 8th 9:30am - 1pm

Solid-state NMR for Protein Structure Determination

Solution NMR of Membrane Proteins

COURSE AIMS

The course will focus on the application of solution-state NMR to the study of different types of biomolecules. The emphasis is on solution-state NMR studies of proteins but applications to nucleic acids and oligosaccharides will also be presented. In addition, more advanced topics including residual dipolar couplings, dynamics, solid-state biomolecular NMR and protein-ligand interations will be introduced.

COURSE FORMAT

Topics to be covered include:

1) Introduction to Biomolecular NMR
2) Assignment of Protein NMR Spectra
3) Assignment using 15N and 13C Labeling
4) Data Harvesting and Structure Determination I
5) Residual Dipolar Couplings
6) Data Harvesting and Structure Determination II
7) Protein Dynamics
8) Using NMR to study Intrinsically Disordered Proteins
9) Using NMR Spectroscopy to Study Protein Folding and Partially Folded Proteins
10) Protein-ligand interactions by NMR
11) Solution NMR Studies of Membrane Proteins
12) Principles and applications of biological solid state NMR
13) Solid-state NMR for protein structure determination
14) NMR of Nucleic Acids
15) Use of NMR for identification and conformational determination of oligosaccharides and related alkaloids

COURSE LENGTH 

The course will run for 5 days and include lectures and practical sessions.

PARTICIPANT NUMBERS 

Maximum 25